Naked DNA approaches have also been explored, especially in the context of vaccine development.
Gene editing is a potential approach to alter the human genome to treat genetic diseases, viral diseases, and cancer.
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The first therapeutic use of gene transfer as well as the first direct insertion of human DNA into the nuclear genome was performed by French Anderson in a trial starting in September 1990.
Between 1989 and February 2016, over 2,300 clinical trials had been conducted, more than half of them in phase I.
The target DNA is then analyzed for the presence of the probe via radioactivity or fluorescence.
In this experiment, as in most molecular biology techniques, a control must be used to ensure successful experimentation.
PCR can also be used to determine whether a particular DNA fragment is found in a c DNA library.
PCR has many variations, like reverse transcription PCR (RT-PCR) for amplification of RNA, and, more recently, quantitative PCR which allow for quantitative measurement of DNA or RNA molecules Allele-specific oligonucleotide (ASO) is a technique that allows detection of single base mutations without the need for PCR or gel electrophoresis.
The reaction is extremely powerful and under perfect conditions could amplify 1 DNA molecule to become 1.07 billion molecules in less than 2 hours.