PCR products of beta-actin were detected from as little as a single cell. Linear and Sensitive Isolation of Both Large and Small RNA. Hill, Michael Milosevic, Fei-Fei Liu Katerina Tomankova, Katerina Polakova, Klara Pizova, Svatopluk Binder, Marketa Havrdova, Mary Kolarova, Eva Kriegova, Jana Zapletalova, Lukas Malina, Jana Horakova, Jakub Malohlava, Argiris Kolokithas-Ntoukas, Aristides Bakandritsos, Hana Kolarova, Radek Zboril Peter L. Deep sequencing combined with microarray hybridization to identify novel and conserved micro RNAs during somatic embryogenesis of hybrid yellow-poplar (Liriodendron chinense (Hemsl.) Sarg.
Five microliters of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using oligo d T primer. Allebrandt KV, Amin N, Müller-Myhsok B, Esko T, Teder-Laving M, Azevedo RV, Hayward C, van Mill J, Vogelzangs N, Green EW, Melville SA, Lichtner P, Wichmann HE, Oostra BA, Janssens AC, Campbell H, Wilson JF, Hicks AA, Pramstaller PP, Dogas Z, Rudan I, Merrow M, Penninx B, Kyriacou CP, Metspalu A, van Duijn CM, Meitinger T, Roenneberg T..
Three microliters of the reverse transcription was used in a 20 µL PCR reaction with primers to detect the human beta-actin transcripts. Bueno C, Montes R, Melen GJ, Ramos-Mejia V, Real PJ, Ayllón V, Sanchez L, Ligero G, Gutierrez-Aranda I, Fernández AF, Fraga MF, Moreno-Gimeno I, Burks D, Del Carmen Plaza-Calonge M, Rodríguez-Manzaneque JC, Menendez P.
Total RNA was isolated from 50, 100 or 200 µL of rat plasma in triplicates using Norgen's Total RNA Purification Kit (blue), a competitors silica-based kit (green) and a phenol-based RNA extraction method (red).
Stem loop RT-q PCR using primers specific to mi R-21 was performed. Hollander Yong H Woo, Hifzur Ansari, Thomas D Otto, Christen M Klinger, Martin Kolisko, Jan Michálek, Alka Saxena, Dhanasekaran Shanmugam, Annageldi Tayyrov, Alaguraj Veluchamy, Shahjahan Ali, Axel Bernal, Javier del Campo, Jaromír Cihlář, Pavel Flegontov, Sebastian G Gornik, Eva Hajdušková, Aleš Horák, Jan Janouškovec, Nicholas J Katris, Fred D Mast, Diego Miranda-Saavedra, Tobias Mourier, Raeece Naeem, Mridul Nair, Aswini K Panigrahi, Neil D Rawlings, Eriko Padron-Regalado, Abhinay Ramaprasad, Nadira Samad, Aleš Tomčala, Jon Wilkes, Daniel E Neafsey, Christian Doerig, Chris Bowler, Patrick J Keeling, David S Roos, Joel B Dacks, Thomas J Templeton, Ross F Waller, Julius Lukeš, Miroslav Oborník, Arnab Pain Mariana Leticia Matias, Mariana Romão, Ingrid Cristina Weel, Vanessa Rocha Ribeiro, Priscila Rezeck Nunes, Vera Therezinha Borges, João Pessoa Araújo, Jr, José Carlos Peraçoli, Leandro de Oliveira, Maria Terezinha Peraçoli Alessandra Ferrajoli, Cristina Ivan, Maria Ciccone, Masayoshi Shimizu, Yoshiaki Kita, Masahisha Ohtsuka, Lucilla D’Abundo, Jun Qiang, Susan Lerner, Nazila Nouraee, Kari G.
Four microliters of the polyadenylated RNA were used in a 20 µL reverse transcription reaction with a poly T adaptor primer. M.; Looijen-Salamon, Monika G.; de Geus-Oei, Lioe-Fee; van der Drift, Miep A.; van der Heijden, Erik H. M.; Oyen, Wim J.; Visser, Eric P.; Span, Paul N.; Bussink, Johan.
One microliter of the reverse transcription was used in a 20 µL q PCR reaction with primers against the human micro RNAs ( He La cells, 100 µL rat blood and 10 mg hamster kidney using Norgen's Total RNA Purification Kit. Teresa Lorenzi, Elena Annabel Niţulescu, Antonio Zizzi, Maria Lorenzi, Francesca Paolinelli, Simone Domenico Aspriello, Monica Baniţă, Ştefania Crăiţoiu, Gaia Goteri, Giorgio Barbatelli, Tommaso Lombardi, Roberto Di Felice, Daniela Marzioni mail, Corrado Rubini, Mario Castellucci.In brief, two microliters of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using mi R-21 stem-loop reverse primer or oligo d T primer. Three microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human mi R-21. Armstrong, Katherine Lau, Juned Kadiwala, Robert Lowe, Annela Seddon, Stephen Mann, J. One hundred nanograms of extracted RNA from each kit was applied to an Illumina micro RNA expression profiling kit. Norgen's Total RNA Purification Kit isolates mi RNA from plasma with better diversity than a leading competitor. Scatter plots display better consistency (better clustering) of replicate signals from Norgen's samples. Total RNA including mi RNA was isolated from 100 µL of plasma using Norgen's Total RNA Purification Kit or 625 µL of plasma using Competitor A's leading mi RNA Kit, and was applied to an NCode expression profiling kit. In brief, one microliter of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using mi R-21 stem-loop reverse primer or oligo d T primer.